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maximum size of insert for pET22, pUC19, and pHIS1525

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#1 goh



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Posted 28 October 2009 - 06:31 AM

Hi, good day to everyone....

Does anyone know what is the maximum size of insert gene that can be cloned into vector of pUC19, pET22, and also pHIS1525.... pHIS1525 is a shuttle vector for e.coli and Bacillus megaterium.... but at present it seems rarely to be used in molecular cloning.... have anyone ever used it before?

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