Storing Purified Proteins
Posted 28 October 2009 - 06:30 AM
Looking for suggestions here.
I'm trying to find something that isn't toxic to help preserve purified proteins that are stored at 4C. I know everyone uses NaN3 for this purpose usually, but I can't/won't. The proteins are stored upto a fortnight at 4C and any preservatives added shouldn't interfere with their function.
Has anyone had experience using commercially available preservatives used in the cosmetic/food industry for such purposes? I'd be interested to hear your thoughts.
Posted 28 October 2009 - 09:24 PM
Yo want to presenrve it in terms of saving it from aggregating or deamidating or oxidising, or you just wanna store it without a contam watever the forms generated??
If used filtered buffers, the protein should be good for a fortnight and you need not add any preservatives!!!
Please clarify if this is what you needed or elaborate your querry!!!
Posted 29 October 2009 - 07:25 PM
I agree to taht too!! Why dint i think of it.. we do it tat way here some times!!!
May be a dull day ahead
Posted 29 October 2009 - 07:55 PM
and also if the impuriteis that you are talking about is aggregation.. then beware.. lyophilization can increase aggregation.. unless less amount is lyophilized!!!
Posted 01 November 2009 - 03:55 PM
Yeah, that works pretty well, but the OP wanted some way to store at 4 ˚C.
Posted 03 November 2009 - 08:11 AM
Edited by mdfenko, 03 November 2009 - 08:12 AM.
genius does what it must
i do what i get paid to do