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MMP Zymography


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#1 jakatta70

jakatta70

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Posted 28 October 2009 - 04:06 AM

Hi all,

Ive a quick question regarding MMP zymography. We incorporate gelatin into the gel and allow the MMPs in the loaded sample to digest the gelatin overnight.

After 15 hours Ive been used to seeing nice strong bands of MMP2 and some less strong bands of MMP9. We have recently bought in new gelatin and made a new solution. However now we dont see any bands! My supervisor assures me this is normal with new gelatin and he seems to be correct as he is able to see his bands on the gel after he stains in and destains it.

I just want to know what makes an "older" batch of gelatin better at visualising bands after 15 hours while "newer" gelatin doesnt reveal anything until the gel is stained.




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