2 replies to this topic

[march]

In real time pcr,we start with the rna sample and use a primer to do the reverse transcription,then for the second step(with the cDNA)can we use the same primer,or we have to design another.
thanks

[Prep!]

march, on Oct 28 2009, 04:40 PM, said:

In real time pcr,we start with the rna sample and use a primer to do the reverse transcription,then for the second step(with the cDNA)can we use the same primer,or we have to design another.
thanks

http://en.wikipedia.org/wiki/Rapid_Amplifi...on_of_cDNA_Ends

[dpo]

I have done a similar experiment and, although it's not a preferred option, sometimes it is necessary cos it's the only place where you can get a specific binding. So my advice would be to give it a try, you already have the primer. If it doesn't work, you can always still order an additional primer.