2 replies to this topic

[abohollolo]

Has anybody tried ELISA for collagen type I using ELISA kit from Chondrex ? i tried like million times and never worked. although i did gel separation i could see the band .
i want to use a new KIT from different company but i want to check before spending all that money .

[facsgirl]

abohollolo, on Oct 27 2009, 09:55 PM, said:

Has anybody tried ELISA for collagen type I using ELISA kit from Chondrex ? i tried like million times and never worked. although i did gel separation i could see the band .
i want to use a new KIT from different company but i want to check before spending all that money .

I'm not familiar with the Chondrex Collagen ELISA, but Cosmo Bio sells a Human Collagen I ELISA. They currently have a promotion for it until the end of the year. Check it out on this link:

http://www.cosmobio.co.jp/export_e/product...CB_20060222.asp

[MD Bioproducts]

MD Bioproducts has a Collagen Type I ELISA (Catalog # M036007)

Specifications:
Species: human
Sample Type: human cells
Sample Preparation: collagen must be solubilized
Sample Size: 100 uL
Standard Curve Range: 0.062 - 5 µg/mL
Sensitivity: 0.35 µg/mL
Assay Length: <3 hrs

http://www.mdbioproducts.com/products/elis...en-type-i-elisa