2 replies to this topic

[abohollolo]

Has anybody tried ELISA for collagen type  I using ELISA kit from Chondrex ?  i tried like million times and never worked. although i did gel separation i could see the band .
i want to use a new KIT from different company but i want to check before spending all that money .

[facsgirl]

abohollolo, on Oct 27 2009, 09:55 PM, said:

Has anybody tried ELISA for collagen type  I using ELISA kit from Chondrex ?  i tried like million times and never worked. although i did gel separation i could see the band .
i want to use a new KIT from different company but i want to check before spending all that money .

I'm not familiar with the Chondrex Collagen ELISA, but Cosmo Bio sells a Human Collagen I ELISA. They currently have a promotion for it until the end of the year. Check it out on this link:

http://www.cosmobio....CB_20060222.asp

[MD Bioproducts]

MD Bioproducts has a Collagen Type I ELISA (Catalog # M036007)

Specifications:
Species: human
Sample Type:  human cells
Sample Preparation: collagen must be solubilized
Sample Size: 100 uL
Standard Curve Range:  0.062 - 5 µg/mL
Sensitivity:  0.35 µg/mL
Assay Length:   <3 hrs

http://www.mdbioprod...en-type-i-elisa