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Immunodepletion of high abundance protein


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#1 anasyida69

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Posted 27 October 2009 - 06:23 PM

Hi there,

I have problems in the reproducibility of the human serum depletion step. Most of the times, high level of albumin was found in low abundant protein (by SDS-PAGE). I use MARS column to deplete 30 samples until now.

Thanks

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Anasyida

#2 mdfenko

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Posted 28 October 2009 - 06:01 AM

have you considered igy based depletion?

check this brochure from beckman-coulter and phenomenex:

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