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How to denaturate protein for ELISA


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#1 Rub

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Posted 27 October 2009 - 08:47 AM

Hi, I have overexpressed my protein in a cell line and have collected the media to study the secretion of my protein. How could i use Urea or guanidine hydrocloride to denaturate my samples before ELISA? Or what other method could I use to denaturate my protein?
Thank you :)

#2 Prep!

Prep!

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Posted 28 October 2009 - 09:25 PM

Usually 6 or 8M urea or Gu HCl shoyld do the trick but I am not sure if it will interfere in the ELISA!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!




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