Posted 27 October 2009 - 08:12 AM
Posted 27 October 2009 - 06:33 PM
Posted 08 January 2010 - 01:01 PM
I got 11 uGg from 4 mL culture.Is that Ok for a low copy plasmidZ?
Posted 10 January 2010 - 12:27 AM
So do not rely to much on those spectrometer readings ...put the DNA on an agarose gel an quantify it there (this way you are able to make some qualitativ statements about your preparation, e.g. is it supercoiled or degraded).
If you really done a miniprep (you stated that you get 50ml of 500ng/µl ...this is not a mini-prep ...thats more a terra-prep
So i think you meant 50µl
As far as i know 25µg is the amount a standard-column can bound ...so this is the maximum amount that you can elute from a column.
Very good prep results i think!
Posted 10 January 2010 - 08:39 AM