Posted 27 October 2009 - 07:15 AM
I am writing a proposal for my preliminary exam and I have a question. I am very new to this field of micro-RNA.
I wanted to know if I tested that my micro-RNA targets a gene in HeLa cells by luciferase assay, will the micro-RNA surely target this gene in my cell line of interest.
In case of HeLa cells, I will be transfecting the synthetic micro-RNA too. However, in my cell line of interest HBMVEC, I know this micro-RNA is expressed. So should I also do a luciferase report analysis in HBMVEC along with Hela cells?
I appreciate your help and time.
Thanks a lot.
Posted 27 October 2009 - 11:05 AM
Posted 27 October 2009 - 11:24 AM
Thanks so much . That answers my question partially. But do you think a micro-RNA targets different genes in different cell lines ?
Posted 27 October 2009 - 11:57 AM
Posted 27 October 2009 - 04:04 PM