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adding koazak sequence to primer

Started by sara.r, Oct 27 2009 06:24 AM

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#1 sara.r

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Posted 27 October 2009 - 06:24 AM

Hi
I know the consensus kozak sequence is gccgcc(a/g)ccAUGg but the second codon in my gene is started with a not g. should I convert it to g to make it identical to kozak or using just gccgcc(a/g)ccAUG is OK?
best

#2 bob1

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Posted 28 October 2009 - 03:14 PM

Don't convert the G, it should be fine without it.
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