I am trying to isolate a plant gene by using degenerate primers designed from conserved consensus of homologous genes from other plants. I was able to get a faint PCR band of expected length (750bp) together with intense primer dimer bands. Upon reamplification, I could not get the amplicon of interest because the primer dimer tends to predominate during reamplification.
What cloning kit would you recommend for faint PCR bands? Thanks in advance!
Submit your paper to J Biol Methods today!
cloning faint PCR bands
No replies to this topic