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cloning faint PCR bands

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#1 kristian



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Posted 27 October 2009 - 04:14 AM

I am trying to isolate a plant gene by using degenerate primers designed from conserved consensus of homologous genes from other plants. I was able to get a faint PCR band of expected length (750bp) together with intense primer dimer bands. Upon reamplification, I could not get the amplicon of interest because the primer dimer tends to predominate during reamplification.

What cloning kit would you recommend for faint PCR bands? Thanks in advance!

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