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low od260/230 for rna


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#1 Vini

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Posted 27 October 2009 - 04:05 AM

hi
can anyone tell me what does a low 260/230 od for RNA mean????
does a high 230 od reflect higher polysaccharide levels in the extracted rna???

thanks

#2 miBunny

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Posted 27 October 2009 - 05:45 PM

absoption at 230 nM is usually an organic contaminants such as thiocyanates or phenolate ions. Did you purify your RNA with a kit? Some of the RNA prep kits with throw in guanidine thiocyanate to prevent DNAse and RNAse activity. This can sometimes show up in the final RNA




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