can anyone tell me what does a low 260/230 od for RNA mean????
does a high 230 od reflect higher polysaccharide levels in the extracted rna???
low od260/230 for rna
1 reply to this topic
Posted 27 October 2009 - 05:45 PM
absoption at 230 nM is usually an organic contaminants such as thiocyanates or phenolate ions. Did you purify your RNA with a kit? Some of the RNA prep kits with throw in guanidine thiocyanate to prevent DNAse and RNAse activity. This can sometimes show up in the final RNA