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[Vini]

hi
can anyone tell me what does a low 260/230 od for RNA mean????
does a high 230 od reflect higher polysaccharide levels in the extracted rna???

thanks

[miBunny]

absoption at 230 nM is usually an organic contaminants such as  thiocyanates or phenolate ions.  Did you purify your RNA with a kit?  Some of the RNA prep kits with throw in guanidine thiocyanate to prevent DNAse and RNAse activity.  This can sometimes show up in the final RNA