tissue culture and its problem???
Posted 27 October 2009 - 01:12 AM
I want to ask u question
I try to do plant tissue culture and every time there is specific type of fungs apper to me even I consider the aseptic condition so the culture failed
How can I solve this problem??
To complete mywork??
Posted 27 October 2009 - 08:49 AM
if you're getting contamination it basically is because your conditions are not sterile enough. the problem you're facing might be due to many different reasons, so try to discard each at a time. first, make sure the tissue you're using has been properly treated (e.g. sodium hypochlorite), also you should do all your tissue culture in a laminar flow cabinet, wear gloves, have a bunsen burner near you and once you start try not to take your hands out and seal everything before you move it outside the cabinet.
hope this helps.
Posted 29 October 2009 - 01:59 AM
Posted 10 February 2010 - 07:46 AM
But be sure you flame all your forceps/blades properly, not just to get rid of bacteria/fungi but also to remove any traces of plant tissue (sometimes callus can get stuck in the grooves of forceps).
Another thing I do is pour all my media at least four days in advance of using it. That way, if it's contaminated I should start to see something growing before I put my plants in there.
Does anyone else have the same problem?
Posted 22 September 2010 - 11:50 PM
Posted 13 October 2010 - 03:50 AM
Posted 13 October 2010 - 07:10 AM
Just my 2 cents.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434