2 replies to this topic

[Xenn]

i use this PCR cloning kit to ligate my DNA.  in the handbook it says like this:

DNA product   4 microlitres
Vector            1 microlitres
master mix     5 microlitres

but i have modified the amount of DNA product and the vector, where the amount is:
DNA product   2 microlitres
Vector            7 microlitres
master mix    ?

How much of master mix should i added in my modification in order to get a successful transformation and it is possible? tq

Edited by Xenn, 27 October 2009 - 05:27 AM.

[laurequillo]

really? are you asking how to do a 1x buffer from a 2x buffer? or maybe I did not understand you...

Edited by laurequillo, 27 October 2009 - 02:59 AM.

[Xenn]

opps. thanks for replying me. here is the new one