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DNA ligation


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#1 Xenn

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Posted 27 October 2009 - 12:08 AM

i use this PCR cloning kit to ligate my DNA. in the handbook it says like this:

DNA product 4 microlitres
Vector 1 microlitres
master mix 5 microlitres

but i have modified the amount of DNA product and the vector, where the amount is:
DNA product 2 microlitres
Vector 7 microlitres
master mix ?

How much of master mix should i added in my modification in order to get a successful transformation and it is possible? tq

Edited by Xenn, 27 October 2009 - 05:27 AM.


#2 laurequillo

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Posted 27 October 2009 - 02:03 AM

really? are you asking how to do a 1x buffer from a 2x buffer? or maybe I did not understand you...

Edited by laurequillo, 27 October 2009 - 02:59 AM.

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#3 Xenn

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Posted 27 October 2009 - 05:28 AM

opps. thanks for replying me. here is the new one




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