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gene copy number calculation


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#1 SF_HK

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Posted 27 October 2009 - 12:05 AM

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?

#2 Prep!

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Posted 27 October 2009 - 12:45 AM

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?


hope this will help u!!!!
find the attachment!!!

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#3 SF_HK

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Posted 27 October 2009 - 11:56 PM

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?


hope this will help u!!!!
find the attachment!!!

Thank you very much




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