Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
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Posted 27 October 2009 - 12:45 AM
SF_HK, on Oct 27 2009, 02:35 PM, said:
Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
hope this will help u!!!!
find the attachment!!!
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Posted 27 October 2009 - 11:56 PM
Pradeep Iyer, on Oct 27 2009, 04:45 PM, said:
SF_HK, on Oct 27 2009, 02:35 PM, said:
Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
hope this will help u!!!!
find the attachment!!!
