Posted 26 October 2009 - 11:53 PM
Posted 27 October 2009 - 12:56 AM
bands smear usually due to 1. too much DNA, 2. DNase presents.
will the electrophoresis band smears when ur PCR product has been reamplified for several times? tq
from my expreience re-amplification with the same primers may yield a very large amount of primer dimer, and if the same time DNase present would have smeared bands (or lanes)