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RNA - bad 230/280 and 260/280 ratio


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3 replies to this topic

#1 moljul

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Posted 26 October 2009 - 11:48 PM

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions

#2 Vini

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Posted 27 October 2009 - 03:03 AM

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions



u can try passing ur samples thru Qiagen column for purification. it SOMETIMES, improves the quality...
i hate 2 say this, but if I were u, i will do the extraction again, coz the ratios are really bad..... :)
all d best :(

#3 Prep!

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Posted 27 October 2009 - 03:32 AM

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions



u can try passing ur samples thru Qiagen column for purification. it SOMETIMES, improves the quality...
i hate 2 say this, but if I were u, i will do the extraction again, coz the ratios are really bad..... :)
all d best :)


hehehe.. me too!!!!
:(
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#4 miBunny

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Posted 27 October 2009 - 05:47 PM

I would go with re-prepping the samples. I have never been able to "clean up" samples that started out looking ugly. It saves time to just start again




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