3 replies to this topic

[moljul]

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions

[Vini]

moljul, on Oct 27 2009, 02:18 PM, said:

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions

u can try passing ur samples thru Qiagen column for purification. it SOMETIMES, improves the quality...
i hate 2 say this, but if I were u, i will do the extraction again, coz the ratios are really bad.....
all d best  

[Prep!]

DRN, on Oct 27 2009, 05:33 PM, said:

moljul, on Oct 27 2009, 02:18 PM, said:

did a series of RNA extraction and one sample had very bad qualitiy 260/280 ratio: 1,14 and 230/280 ratio: 0,99!

what can i do to improve the quality of this sample prior to reverse transcription??

thanks for suggestions

u can try passing ur samples thru Qiagen column for purification. it SOMETIMES, improves the quality...
i hate 2 say this, but if I were u, i will do the extraction again, coz the ratios are really bad.....
all d best  

hehehe.. me too!!!!

[miBunny]

I would go with re-prepping the samples.  I have never been able to "clean up" samples that started out looking ugly.   It saves time to just start again