18 replies to this topic

[fortunate]

I have 50 mice for genotyping. But I don't know how to mark mice for my expirement.
What I am using is make lines on the tail with a marker, but it only lasts for 3 days.
Anyone can tell me the standard method, punch ears, cut nails?
thanks!

[Prep!]

fortunate, on Oct 27 2009, 11:00 AM, said:

I have 50 mice for genotyping. But I don't know how to mark mice for my expirement.
What I am using is make lines on the tail with a marker, but it only lasts for 3 days.
Anyone can tell me the standard method, punch ears, cut nails?
thanks!

some people punch ears... others mark them with permenant dye colors!!!
some others shave on different regions... (Tricky.. need to keep track)

[fortunate]

some people punch ears... others mark them with permenant dye colors!!!
some others shave on different regions... (Tricky.. need to keep track)
[/quote]


You are helpful, but I would be grateful if you explain more or provide a link that I can refer to.

[Prep!]

try these if they help

http://www.researcht...=606#lesson7728

http://vision.ucsd.e...audBelongie.pdf

Best luck!!!

[Fungus_Dreams]

I use ear punching. You're going to need to be trained by someone, do you have a professor or TA who could show you? If not, one of your facility's Animal Care Technicians may be able to help you if you ask.

Here's the 'standard' way to ear punch mice, but you can use your own variation. http://www.bu.edu/re...-punching.shtml



In my experience, It's easier to number a few mice in each cage, rather than try to accurately punch every number 1-99. What I'd do is: say you have a cage with 5 mice, mark them NM (no mark, no punch), R (1 hole in right ear), L (1 hole in left ear), RR (2 in right ear), LL (2 in left ear). So your cage would look like this:

NM
R
L
RR
LL

... a cage with 2 mice in it would just be NM, R, make sense? Hope this was helpful I love working with my mice.

[fortunate]

Thank you guys, that's exactly what i need!

[karljoesen]

I think you should catch a mouse that have a signal with a piece of paper round its neck

[Rupam]

You can number the mice and put them in different colour trays which will give you the account of how many mice about to go and how many you had done with.
I hope this will help.

You can learn about the more scope in this field by referring [url="[url]http://www.wiziq.com/tutorial/22753-5-Scope-of-biology"]scope[/url] of biology[/url] from wiziq.

Regards
Rupam

[nightingale]

we use a permanent dye, to color the tails.
and numbering or writing valuable info. on the tray itself, works fine too.

[toxo]

Here is an alternative method that has worked very well.

Mouse Ear Tattoo

Edited by toxo, 18 November 2010 - 12:00 PM.

[Say Chi Sin Lo]

Why not just ear-tag them? Be careful though, if they're not on properly (away from the edge of the ear), they tend to fall off.

[girlxinh]

you do earrings and paper to mark number to each











dich thuat | du hoc anh | hoc bong du hoc

[Fungus_Dreams]

Personally I'm not a fan of the ear tags, as they can tear out. At best you get a little mouse with a big, awkward looking tag flopping around.

We recently tried the mouse tattoo kit in my lab with great success. So far we've only tried doing mouse toes.... waiting to get committee approval for mouse ears  
But I think inking ears will be a further improvement over toes, since you won't have to pick up each animal and examine its feet.

[Habbakuk]

I don't like to use ear tags, it feels cruel. I usually separate them accordingly.
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[adibah]

i've tried by staining with picric acid. its supposed to be in powder form. But the one I have is in solution. What I did was I dip cotton bud into the picric acid, and stain the mice according to my number coding (right front hand = 1, left front hand = 2, right flank = 3, left flank = 4, head =5). I tried this last 2 weeks, and the dye still there and all the mice are ok (no sign of abnormality). just make sure they don't lick the dye before the dye dried. you can apply it by holding the mice by tail.

hope it helps! =)