Hello everyone
I'm having a bit of trouble establishing primary muscle cultures and wondered if anyone could help.
Basically, I've developed a protocol for isolating the hind limb muscles from neonate mice, enzymatically digesting them and plating them out. I then have trouble keeping them at the right confluency - too dense and they differentiate, too sparse and become senescent!!! It seems to be an incredibly delicate balance.
Does anyone have any muscle/myoblast culturing experience - I could do with some hints and tips on how to keep them growing!
BW
Emily
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