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2D gel


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#1 rijomarmi

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Posted 26 October 2009 - 12:00 PM

Which is the maximum amount of protein that can be loaded in 11 cm IPG strips? Does anyone can hepl me?
thanks!

#2 fishdoc

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Posted 26 October 2009 - 12:30 PM

Which is the maximum amount of protein that can be loaded in 11 cm IPG strips? Does anyone can hepl me?
thanks!



I don't know the max, but I frequently ran 100 ug of bacterial whole cell lysate protein. Most spots separated well, but a few of the housekeeping proteins were pretty big spots, which may have masked other spots. Had pretty similar results with 50 and 75 ug, too.




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