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HL-60 cultures


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#1 bcsein

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Posted 26 October 2009 - 10:19 AM

Hi,
I am planning to use HL-60 cell cultures and I realized there are differences in culture conditions , for example medium RPMI in some labs has 5% FBS , in others it has 10% FBS.

Can anybody advice the better way to culture them, also to freeze them? Which desity should I use and how many days I should wait to make subculturing?

Thank you very much

#2 refolder

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Posted 13 November 2009 - 04:58 AM

Dear bcsein,


Simply culture your cells with 5% or 10% FBS, and monitor the cell growth. Usually 10% is better.

For freezing, 10% DMSO with 10 - 20% FBS + RPMI (or DMEM-F12, other cell media I use) works well.




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