Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

protein size change after western blot

  • Please log in to reply
1 reply to this topic

#1 glbinbin



  • Members
  • Pip
  • 4 posts

Posted 26 October 2009 - 07:53 AM

My target protein is about 61KD.
But I did the western blot, the band was between 45KD~55KD.
What's the matter? How to explain it?Please give me some advice. Thanks a lot!

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,268 posts

Posted 26 October 2009 - 08:27 AM

proteins don't always migrate to the proper molecular weight. sds-page is not perfect for mw determination. it gives a good approximation but there are a number of factors that may alter mobility.

you should confirm with other techniques (eg gel filtration).
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.