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protein size change after western blot


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#1 glbinbin

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Posted 26 October 2009 - 07:53 AM

My target protein is about 61KD.
But I did the western blot, the band was between 45KD~55KD.
What's the matter? How to explain it?Please give me some advice. Thanks a lot!

#2 mdfenko

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Posted 26 October 2009 - 08:27 AM

proteins don't always migrate to the proper molecular weight. sds-page is not perfect for mw determination. it gives a good approximation but there are a number of factors that may alter mobility.

you should confirm with other techniques (eg gel filtration).
talent does what it can
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