i've an unusual question to ask. 99% of my job is real time PCR. I have my Cts, my normalizer and so on. i calculate DCt by subtraction of Ct sample - Ct normalizer. i then compare groups using DDCt method (DCt of sample-DCt control) and with the 2

^{-DDCt}i have my fold induction.

Question is how do u calculate statistics? i mean do you calculate significativity among groups (treated Vs control) using the DCt s or with folds? same thing for SEM or SD?

i always do my statistics on DCts but now somebody tells me i have to do it on FOLDs. I'm getting confused....

thanks