DNA binding/EMSA question
Posted 25 October 2009 - 09:20 AM
Posted 25 October 2009 - 02:50 PM
Posted 27 October 2009 - 03:26 PM
You've established that protein A binds DNA and protein B does not; you have not, however, established that this is in any way sequence specific. It would be most valuable to test the binding of protein A against one or a few randomly permuted sequences to verify that it does not bind. The competitive binding experiment would also be good, but again you should be using both sequence A and a randomized sequence as competitors.
Thanks for the reply, it was very informative. I see the distinction now. I have have a followup question on the randomized/scramble sequences. Is there a logic to choosing the scramble sequence? Some papers I've read only change a couple of bases in the consensus region while others make it completely different.
Posted 27 October 2009 - 06:07 PM
Posted 27 October 2009 - 09:35 PM
"This is SPARTA!"
"I´m the goddamn batman"