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protein expression (temperature issue)


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17 replies to this topic

#16 bhavana

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Posted 11 September 2010 - 09:06 AM

cn u say what's ur protein size.

#17 Carmela

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Posted 06 December 2010 - 03:50 PM

Use a tag!
If you are having that kind of problems it's the best way: it's easy to clone, easy to identify... and easy to remove...
HPLC, mass spectrometry, western, ELISA....c'mon... each time? Add any tag (6xhis?) and if you need it, you can remove it later... but maybe you don't need it!

#18 Adrian K

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Posted 06 December 2010 - 08:24 PM

Not sure whether is it relevant but you can try cell free expression...
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

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"It's all just DNA. Do it."---phage434




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