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Is Taq polymerase still active after staying at 10C for one day?


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2 replies to this topic

#1 DigitalPig

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Posted 24 October 2009 - 06:49 PM

I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!

#2 perneseblue

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Posted 24 October 2009 - 06:56 PM

I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!


Yes. Give it a try. It should be okay from this miss adventure.
May your PCR products be long, your protocols short and your boss on holiday

#3 phage434

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Posted 24 October 2009 - 06:56 PM

Yes, almost certainly. Taq is a high temperature enzyme which survives 95C in a thermocycler. The main danger would be bacterial or fungal growth, but this is unlikely over a day, and probably would be inhibited by other components of the enzyme buffer.




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