I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!
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Is Taq polymerase still active after staying at 10C for one day?
Started by DigitalPig, Oct 24 2009 06:49 PM
2 replies to this topic
#2
perneseblue
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Unlimited ligation works!
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Posted 24 October 2009 - 06:56 PM
DigitalPig, on Oct 24 2009, 06:49 PM, said:
I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!
Yes. Give it a try. It should be okay from this miss adventure.
May your PCR products be long, your protocols short and your boss on holiday
#3
phage434
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Posted 24 October 2009 - 06:56 PM
Yes, almost certainly. Taq is a high temperature enzyme which survives 95C in a thermocycler. The main danger would be bacterial or fungal growth, but this is unlikely over a day, and probably would be inhibited by other components of the enzyme buffer.
