MDCK cells vacuolation
Posted 24 October 2009 - 06:42 PM
I borrowed MDCK cells from my prof, and I started culturing them from passage 25 to passage 35, I am using them for viral studies.
When i stain the cells i see vacuolation. This is what I do,
Grow them on slides for 24-32 hours, wash them with PBS pH 7.2
Then fix them using 80% acetone or 80% methanol,
Then stain them using hematoxylin then eosin with steps involving dehydration, I see vacuolation even before dehydration using ethanol.
THis means that there is problem in the cells can someone helo me out with this problem, what should I do with the vacuolation?
I use DMEM as growth medium and have dissolved ampicillin.
Posted 26 October 2009 - 03:41 PM
Posted 27 October 2009 - 09:31 PM
i will attach two of them, pls have a look. I will attach few more in sometime.
Please comment on them
Posted 28 October 2009 - 03:20 PM
Posted 28 October 2009 - 05:08 PM
the link above is to the ATCC which has a picture and their recommendations for culture conditions.
Do you have any from an lower passage number ?- you have got a fairly high passage number there.maybe try a "younger" cell and see if you have the same problems.