seperating cells and antibodies
Posted 24 October 2009 - 06:26 PM
At what speed should I run a centrifuge to separate cells secreting antibodies. Is 1000g at 5 mins enough to separate cells from the antibodies
Please reply...Also if you can send me the link where this procedure is mentioned, it will be helpful
Posted 27 October 2009 - 09:35 PM
Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.