2 replies to this topic

[miraclestrain]

Hi,

At what speed should I run a centrifuge to separate cells secreting antibodies. Is 1000g at 5 mins enough to separate cells from the antibodies

Please reply...Also if you can send me the link where this procedure is mentioned, it will be helpful

thank you

MS

[CellSpecific.com]

Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.

[miraclestrain]

Thank you for the tips, i will work on it.

CellSpecific.com, on Oct 26 2009, 03:00 PM, said:

Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.