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seperating cells and antibodies


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#1 miraclestrain

miraclestrain

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Posted 24 October 2009 - 06:26 PM

Hi,

At what speed should I run a centrifuge to separate cells secreting antibodies. Is 1000g at 5 mins enough to separate cells from the antibodies

Please reply...Also if you can send me the link where this procedure is mentioned, it will be helpful

thank you

MS

#2 miraclestrain

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Posted 27 October 2009 - 09:35 PM

Thank you for the tips, i will work on it.


Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.






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