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Concatemerization of miRNA...


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#1 miRNA_diabetes

miRNA_diabetes

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Posted 23 October 2009 - 12:51 PM

I get 2 bands on agarose gel on running my qPCR products for some miRNAs whereas a few others show just one band around 25bp(expected size). I believe the ones for 2 bands are mature and precursor miRNAs. Has anyone seen concatemerization of some miRNAs whereas some others not showing this?? :D

Thanks!!

#2 Functional Screens

Functional Screens

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Posted 23 October 2009 - 01:56 PM

I wonder which realtime PCR kit you used. Should you get 100-150 bp band?
I don't think 25bp should be the expected/correct size.




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