Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Separation of viral RNA from host nucleic acids

  • Please log in to reply
No replies to this topic

#1 ShannonJ



  • Active Members
  • Pip
  • 24 posts

Posted 23 October 2009 - 08:16 AM

My background is in environmental bacteriology so my apologies is this is a silly question...

I am trying to shot-gun sequence (Illumina/454) RNA viruses. So far I've been able to generate "high-quality" cDNA and from that "very nice" libraries for each of the platforms, but the resulting data is a bit crappy in that there's >99% host sequence resulting in low coverage of the target (viral) genomes. I'm wondering if there's a way (sucrose of CsCl gradient? biotinylated probes attached to magnetic beads?) that we can use to reduce the amount of host signature in the samples.

Preferably this method would use either a cell lysate (we receive them in Trizol) or extracted RNA, rather than an upstream process...but if need be that might be do-able as well.

Thanks in advance!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.