I am extracting DNA from cattle semen straws and I've observed that the yield varies a lot -from 10ng/µl to 150-200ng/µl in a 200µl solution (nanodrop measured). I use the Maxwell16 extractor for this purpose.
it seems like there is differences in straw content, both in viscosity, color (white vs yellow) and "pelletability" during centrifugation/washing so it is very hard to design one universal protocol that always works out fine, both for concentration but also for 260/280 and especially 260/230. 260/230 is often much lower than 1,7 -it might be above 1,0.
Has anybody got a nice protocol that gives both good yields and close-to-optimal ratios? is there any way to improve a 260/230 ratio after extraction, like DNA precipitation and resolving in storage buffer/TE/dH2O?
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