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phenol ph

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#1 tingtong



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Posted 23 October 2009 - 03:48 AM

i have to do dna extraction using phenol-chloroform. phenol tht we have in lab is ph 4. how do i make a solution of ph 8 for this purpose ;) ? pls help me. thnks in advance :P

#2 mdfenko


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Posted 23 October 2009 - 08:17 AM

you can find the methods here on protocols online. this link is to procedures:

molecular biology procedures

to prepare phenol, equilibrate with 1M tris, pH 8. in your case, you may want to first equilibrate with dh2o then with 1M tris.
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