plants bioactive compounds consistancy
Posted 22 October 2009 - 11:34 PM
i am dealing on isolation of antimicrobial bioactive compounds from plant. however, through out my study i found that responsible bioactive compounds from the bioactive fraction always different in different batch of sample collection. so it is so difficult to hv a consistant bioactive supply. Anyone of you hving experiences on these and how to mangange this kind of problem? what the variable that should be taken more in order to hv repeatabe bioactive pattern in the bioactive fraction?
thank for your help guys~~~
Posted 26 October 2009 - 03:51 PM
-is there some factor that could trigger the production of the antibiotic? e.g. bacterial insult of the plant, temperature, humidity, time of day/year... etc.
Then you can get into the sample preparation - same conditions all the time? Do you take the same amount of time each time you extract? Does the compound degrade? Is it a mix of substances rather than just one compound?
Posted 26 October 2009 - 05:39 PM
So aside from sampling the plant at the same time, under the same conditions, you might want to look at propagating some clones/cuttings
Posted 26 October 2009 - 09:00 PM
Posted 27 October 2009 - 07:21 AM
if you want batch to batch uniformity then you will have to collect each batch at the same time of year.
or you can grow the plants in a controlled and unvarying environment.
genius does what it must
i do what i get paid to do
Posted 28 October 2009 - 07:15 AM
however, my research will more emphasis on the bioactive compounds where these is too many thing to take care of in controling these kind of viarable... even next year it cant be garantee that would hv the same weather and more importantly thr microenvironment....
so thinking to discuss see any solution that can be used to minimize the viarable itself~~~ the only solution i hv thought so far is i collect a bulk of sample and do the extraction at once to yield lot of extracts and further it down~~~