5 replies to this topic

[BJSAu]

Hi everyone,
people in my division are using DGGE to separate out PCR sequences after using degenerate primers.  The silver staining technique we use is not optimal, nor has ethidium bromide been all that good. Any suggestions on using silver staining and DGGE in general ?

[taggart]

hi

Are you getting any visible bands using silver staining?  I got bands by staining with ethidium bromide for 10 mins, but am having no luck using silver stain

[Nelson Lab]

SYBR green works much better than Ethidium bromide for me, I have a mixed template from soil, with degenerate primers as well.  It is much more sensitive - I let it stain for 40 minutes.

[taggart]

Hi

I have started to use sybr gold to stain my gels, I stain them for about 15mins this gives a good result, it is also possible to reuse the stain

[Dnase]

I got bands by staining with silver, very well

[Patrick Laplante]

I just tried Sybr Gold.
It's working pretty well.
Patrick