Storing blood to be used for culturing bacteria
Posted 22 October 2009 - 03:55 PM
1. How long will the bacteria remain viable at -70C, -20C, and 4C?
2. What is the best temperature for long-term storage?
Thanks so much!
Posted 23 October 2009 - 12:56 AM
you want to store the bacteria in the blood ? Or?
Or you are going to store the bacteria seperated from the blood?
Posted 23 October 2009 - 08:34 AM
Posted 26 October 2009 - 01:03 AM
If you are going to store the bacteria in a special -80°C freezer (glycerol stock), you should first snap-freeze the bacterial stock by dropping it in a container of liquid nitrogen. If you are storing the bacteria in a regular -20°C freezer, the bacterial stock can be placed there with no further treatment.
Lyophilization is another good means of long term storage (with DMSO)
Only think that i am not sure of is how good blood behaves in a freezer cause normally blood is fractionated and then stored in freezers or cold rooms.