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Storing blood to be used for culturing bacteria


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#1 jeniver

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Posted 22 October 2009 - 03:55 PM

I'm having some trouble finding info on storing blood samples that will be used for culturing bacteria.

1. How long will the bacteria remain viable at -70C, -20C, and 4C?
2. What is the best temperature for long-term storage?

Thanks so much!

#2 pito

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Posted 23 October 2009 - 12:56 AM

I do not follow you completely,

you want to store the bacteria in the blood ? Or?

Or you are going to store the bacteria seperated from the blood?

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 jeniver

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Posted 23 October 2009 - 08:34 AM

Sorry, let me clarify. The bacteria are inside the blood cells, so I will be culturing whole blood. I am wondering how long I could keep these blood samples at these temperatures. For example, if I left the blood samples at 4C for a week, would the bacteria be dead?

#4 Prep!

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Posted 26 October 2009 - 01:03 AM

Wont the blood clot?? (i presume it has been treated with anto clotting agents) :P

If you are going to store the bacteria in a special -80C freezer (glycerol stock), you should first snap-freeze the bacterial stock by dropping it in a container of liquid nitrogen. If you are storing the bacteria in a regular -20C freezer, the bacterial stock can be placed there with no further treatment.
Lyophilization is another good means of long term storage (with DMSO)

Only think that i am not sure of is how good blood behaves in a freezer cause normally blood is fractionated and then stored in freezers or cold rooms.
Support bacteria - They are the only culture some people have!!!
Cheers!!!




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