Storing blood to be used for culturing bacteria
Posted 22 October 2009 - 03:55 PM
1. How long will the bacteria remain viable at -70C, -20C, and 4C?
2. What is the best temperature for long-term storage?
Thanks so much!
Posted 23 October 2009 - 12:56 AM
you want to store the bacteria in the blood ? Or?
Or you are going to store the bacteria seperated from the blood?
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 23 October 2009 - 08:34 AM
Posted 26 October 2009 - 01:03 AM
If you are going to store the bacteria in a special -80°C freezer (glycerol stock), you should first snap-freeze the bacterial stock by dropping it in a container of liquid nitrogen. If you are storing the bacteria in a regular -20°C freezer, the bacterial stock can be placed there with no further treatment.
Lyophilization is another good means of long term storage (with DMSO)
Only think that i am not sure of is how good blood behaves in a freezer cause normally blood is fractionated and then stored in freezers or cold rooms.