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Another band after plasmid purificaiton


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#1 tantao

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Posted 21 October 2009 - 09:26 PM

Dear all,
I have got a problem about plasmid purificaiton. After the plasmid purificaiton, I ran plasmid DNA on a gel , but there is an another band before super colied plasmid .
I do not know what happened . Do you have any suggestions? Thanks a lot!

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  • plasmid_problem.jpg

Edited by tantao, 21 October 2009 - 11:40 PM.


#2 leelee

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Posted 21 October 2009 - 10:04 PM

I'm assuming it is uncut plasmid? In which case it is probably your plasmid in another conformational state, like relaxed circular.......which runs slower than the supercoiled.

#3 tantao

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Posted 21 October 2009 - 11:50 PM

I'm assuming it is uncut plasmid? In which case it is probably your plasmid in another conformational state, like relaxed circular.......which runs slower than the supercoiled.


Dear leelee,
maybe I do not explain this problem clearly. so I edit my photo, and the stranger band, I have marked it with asterisk.. I do not digest the plasmid with any enzyme, there should be no band! so I do not know what happened!




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