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DMEM vs RPMI for primary mouse lymphocytes


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#1 smanivannan

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Posted 21 October 2009 - 02:23 PM

Hey guys,

Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!

-S

#2 bob1

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Posted 21 October 2009 - 03:50 PM

either should be fine, you will need to add lots of serum and probably some growth factors.

#3 Clare

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Posted 22 October 2009 - 01:49 AM

Hello <_<

My recipe for mature B cell media:

RPMI + 50um B-mercaptoethanol, 10% FCS, 10mM HEPES pH 7.4, 0.1mM non-essential amino acids, 2mM L-Glutamine, 1% sodium pyruvate

Hope this helps :blink:

Clare



Hey guys,

Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!

-S






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