Hey guys,
Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!
-S
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2 replies to this topic
#2
bob1
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Posted 21 October 2009 - 03:50 PM
either should be fine, you will need to add lots of serum and probably some growth factors.
#3
Clare
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Posted 22 October 2009 - 01:49 AM
Hello 
My recipe for mature B cell media:
RPMI + 50um B-mercaptoethanol, 10% FCS, 10mM HEPES pH 7.4, 0.1mM non-essential amino acids, 2mM L-Glutamine, 1% sodium pyruvate
Hope this helps
Clare
My recipe for mature B cell media:
RPMI + 50um B-mercaptoethanol, 10% FCS, 10mM HEPES pH 7.4, 0.1mM non-essential amino acids, 2mM L-Glutamine, 1% sodium pyruvate
Hope this helps
Clare
smanivannan, on Oct 21 2009, 11:23 PM, said:
Hey guys,
Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!
-S
Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!
-S
