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[jopa]

Hi,

I'm doing some RNAi experimets with the malaria mosquite, and I have some doubts.

I'm using dsRNA targeted for a couple of genes, my question: is this dsRNA stable for a couple of days, or is it all degraded to siRNA by DICER, I'm asking this because I have some primers that anneal in the same region of the gene and I afraid of getting wrong expression levels if I use these in qRT-PCR to asses the efects of silencing. because I might be also getting amplification of the dsRNA injected

thanks in advance