Warning: array_merge(): Argument #2 is not an array in /home/protocol/public_html/forums/admin/sources/classes/text/parser.php on line 1074

Warning: array_merge(): Argument #2 is not an array in /home/protocol/public_html/forums/admin/sources/classes/text/parser.php on line 1074

Warning: mb_substr_count() expects at most 3 parameters, 4 given in /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php on line 1269

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 110

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 127

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 136

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 137

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser.php:1074) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 141
DNA extraction from Mycobacterium tuberculosis - Molecular Biology - BioForum

Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA extraction from Mycobacterium tuberculosis


  • Please log in to reply
2 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 21 February 2001 - 10:00 PM

> Dear Sir and Madam>

What is the best way of a DNA extraction from Mycobacterium tuberculosis ?> If you have an any experience let me know the method.

Thanks in advice.

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 24 February 2001 - 10:00 PM

You must either spheroplast mycobacterial cells by adding 10 g Glycine per liter of culture at OD 3.0 and letting grow for another 24 hours or by using French Press at 14000 Psi.

Good Luck

DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundation[url="http://www.dstf.technologist.com"][url]http://www.dstf.technologist.com[/url][/url]


#3 123blockhead

123blockhead

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 20 April 2011 - 01:34 AM

You must either spheroplast mycobacterial cells by adding 10 g Glycine per liter of culture at OD 3.0 and letting grow for another 24 hours or by using French Press at 14000 Psi.<p>Good Luck<p>DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundationhttp://www.dstf.technologist.com


Any other efficient way to disrupt Mycobacterium tuberculosis cell wall during DNA extraction?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.