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DNA extraction from Mycobacterium tuberculosis


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#1 anonymous

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Posted 21 February 2001 - 10:00 PM

> Dear Sir and Madam>

What is the best way of a DNA extraction from Mycobacterium tuberculosis ?> If you have an any experience let me know the method.

Thanks in advice.

#2 anonymous

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Posted 24 February 2001 - 10:00 PM

You must either spheroplast mycobacterial cells by adding 10 g Glycine per liter of culture at OD 3.0 and letting grow for another 24 hours or by using French Press at 14000 Psi.

Good Luck

DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundation[url="http://www.dstf.technologist.com"][url]http://www.dstf.technologist.com[/url][/url]


#3 123blockhead

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Posted 20 April 2011 - 01:34 AM

You must either spheroplast mycobacterial cells by adding 10 g Glycine per liter of culture at OD 3.0 and letting grow for another 24 hours or by using French Press at 14000 Psi.<p>Good Luck<p>DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundationhttp://www.dstf.technologist.com


Any other efficient way to disrupt Mycobacterium tuberculosis cell wall during DNA extraction?




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