DNA extraction from Mycobacterium tuberculosis
Posted 21 February 2001 - 10:00 PM
What is the best way of a DNA extraction from Mycobacterium tuberculosis ?> If you have an any experience let me know the method.
Thanks in advice.
Posted 24 February 2001 - 10:00 PM
DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundation[url="http://www.dstf.technologist.com"][url]http://www.dstf.technologist.com[/url][/url]
Posted 20 April 2011 - 01:34 AM
You must either spheroplast mycobacterial cells by adding 10 g Glycine per liter of culture at OD 3.0 and letting grow for another 24 hours or by using French Press at 14000 Psi.<p>Good Luck<p>DianeTechnical Support for Tuberculosis ResearchDr. Shankar's Technology Foundationhttp://www.dstf.technologist.com
Any other efficient way to disrupt Mycobacterium tuberculosis cell wall during DNA extraction?