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elution retarded band

Started by anonymous, Mar 22 2001 10:00 PM

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#1 anonymous

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Posted 22 March 2001 - 10:00 PM

I have been trying to purify a membrane bound protein. It has 5-6 transmembrane segments and very little that is soluble. It is a b-type cytochrome. My efforts to date have failed. I have been trying triton X-114,X100, and Tween 20 in different orders and concentrations and the end result is a non-useable product.

HELP!!!

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