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P1 PHAGEMID dsDNA isolation


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#1 anonymous

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Posted 02 March 2001 - 10:00 PM

From: Alberto
email: visintia@exchange.nih.gov
Category: RNA-Related

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hi there,

i appreciate if anybody with any successful experience in doing reverse northernscould provide me a reliable protocol for labeling cDNA (i use to use superscriptII) orshare with me his/her skills! thank you in advance, Alberto


#2 anonymous

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Posted 03 April 2001 - 09:00 PM

Hi everybody,

I have done recently a subtractive library and I am trying to verify the clones with Reverse Northerns. Unfortunately with little success.I have a lot of troubles and I think the problem is the cDNA labeling.Does anybody has any suggestions? Is it to much if I ask for reliable protocol for labeling cDNA(radioactive or non-radiactive)?I would very much appreciate any help!

Kosmas





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