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Co-transfection of miRNA and vector expressing target sequence


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#1 miRNA_diabetes

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Posted 20 October 2009 - 01:00 PM

Hi,

I am new to this forum and the research field. I would appreciate if someone could help me with this query. I want to do a co-transfection of miRNA with a vector expressing a target sequence? Could someone help with the ratio of miRNA:vector DNA or suggest where I could find it? I am using Metafectene Pro as the transfection reagent in a 6-well plate.

Thank you! ;)

#2 k0k0

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Posted 26 October 2009 - 10:06 AM

depends of the cell type....what kind of cells you try to silencing?

#3 miRNA_diabetes

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Posted 29 October 2009 - 12:37 PM

Hepatoma cell line.. and then eventually primary hepatocytes..




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