2 replies to this topic

[miRNA_diabetes]

Hi,

I am new to this forum and the research field. I would appreciate if someone could help me with this query. I want to do a co-transfection of miRNA with a vector expressing a target sequence? Could someone help with the ratio of miRNA:vector DNA or suggest where I could find it? I am using Metafectene Pro as the transfection reagent in a 6-well plate.

Thank you!

[k0k0]

depends of the cell type....what kind of cells you try to silencing?

[miRNA_diabetes]

Hepatoma cell line.. and then eventually primary hepatocytes..