Hi,
I have 50 micrograms of a protein, of which I want to take 10 micrograms. My best scale is accurate to 0.01 milligram. I don't want to dilute everything at the same time since the expire time will shorten when diluting it and the protein is very expensive. Does anyone have any advice how I should do this?
Thanks!
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#2
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an elder
Posted 20 October 2009 - 06:44 AM
is your balance "accurate" or "precise" to 0.01mg?
if it is "accurate" then you can weigh out 10ug with some confidence. if it is "precise" then i wouldn't consider weighing the protein.
is this a purchased protein? if so then it is usually accompanied by other solids of which the protein is a percentage of the total. in this case you can weigh out the protein and accompanying solids with confidence.
otherwise you will have to bite the bullet and dissolve the whole sample and pipette the amount you need.
if it is "accurate" then you can weigh out 10ug with some confidence. if it is "precise" then i wouldn't consider weighing the protein.
is this a purchased protein? if so then it is usually accompanied by other solids of which the protein is a percentage of the total. in this case you can weigh out the protein and accompanying solids with confidence.
otherwise you will have to bite the bullet and dissolve the whole sample and pipette the amount you need.
Edited by mdfenko, 20 October 2009 - 08:22 AM.
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genius does what it must
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#3
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Veteran
Posted 20 October 2009 - 01:50 PM
Also, if it was bought, ask the company about ways to stabilise it after diluting it.
Be nice to your bureaucrats: they control your budgets...
