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MBD2 and MBD3 ChIP

Started by SIGNY, Oct 20 2009 04:02 AM

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#1 SIGNY

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Posted 20 October 2009 - 04:02 AM

Hi everyone,

I'm new to this forum and at the moment I'm trying to get ChIP working with antibodies against MBD2 and MBD3 (of the NuRD complex). Has anyone of you tried this and can recommend an antibody? I already searched literature but even with antibodies used in ChIPs I don't get any signal. Is it for example better to leave out the LiCL buffer? Or is there any "trick" regarding this two proteins?

Thanks for your help

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#2 KPDE

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Posted 20 October 2009 - 08:46 AM

SIGNY, on Oct 20 2009, 05:02 AM, said:

Hi everyone,

I'm new to this forum and at the moment I'm trying to get ChIP working with antibodies against MBD2 and MBD3 (of the NuRD complex). Has anyone of you tried this and can recommend an antibody? I already searched literature but even with antibodies used in ChIPs I don't get any signal. Is it for example better to leave out the LiCL buffer? Or is there any "trick" regarding this two proteins?

Thanks for your help

Have you gotten ChIP to work with any other antibodies for other proteins, for instance Pol II or histones?

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