what primers should I use for DNA sequencing? please help
Posted 19 October 2009 - 05:42 AM
Thank you all so so much!!
Posted 19 October 2009 - 07:13 AM
Posted 19 October 2009 - 02:09 PM
I can't find a pED-3D vector but there is a pET-3D vector - is that it? Why don't you know your insertion site? Looking at the vector map, you could try T7 but if that's not near where your insertion is it won't do you any good. Otherwise I guess you could get the vector sequence and design some primers at various points around the vector near where insertion sites could be and just keep trying.
thanks, I will try to find more info on the insertion site.....