Jump to content


- - - - -

Creating stable cell lines with pT-Rex-DEST30


No replies to this topic

#1 DavidGA

    member

  • Members
  • Pip
  • 1 posts

Posted 19 October 2009 - 01:08 AM

Hello all,

I have used the Invitrogen pT-Rex-DEST30 to create stable cell lines. However, I did not co-transfect with the pcDNA6/TR vector that expresses the TetR protein for the Tet system.
My question is:
Will the protein of interest sill express even if the TetR protein (repressor) is not present?
Many thanks for your help,

David





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.