Excuse me! I have a question. You mean that I should jsut add the matrigel on the filter followed by removing it. Could the matrigel be coated on the filter by this method? I am afraid of all the matrigel being removed from the filte! Would there be an enough amount of matrigel remaining on the filter? Thanks for reply!
amelodie, on Oct 22 2009, 05:08 AM, said:
This is the protocol we are using in the lab: matrigel is diluted 1:1 in IMDM, aliquoted and stored at -20C.
Aliquots are thawed overnight on ice before use. Before coating, we are storing the plates/filters on ice to have them ice-cold. Then we are just adding the matrigel, removing the excess and leave the plates flat and untouched on ice for at least 15 min. After that, just pipette the excess of matrigel if any, let rest 5 more min on ice, 5 min at RT and use.
Never had problem like that, hope it will work for you,
Edited by icome, 25 October 2009 - 09:23 PM.