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Antibody titration and Cell number


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#1 borriello.87

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Posted 18 October 2009 - 04:00 AM

Hi all,
I have to titrate some fluorochrome-conjugated antibodies for flow cytometry experiments and I'd like to solve some doubts.
If I titrate an antibody with a cell concentration of 2*10^5 cell/100ul, do I need to titrate it again when using a cell
concentration of 5*10^5-10^6 cell/100ul?

Thanks a lot!

#2 pesji

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Posted 20 October 2009 - 01:55 AM

Hi all,
I have to titrate some fluorochrome-conjugated antibodies for flow cytometry experiments and I'd like to solve some doubts.
If I titrate an antibody with a cell concentration of 2*10^5 cell/100ul, do I need to titrate it again when using a cell
concentration of 5*10^5-10^6 cell/100ul?

Thanks a lot!


I don't think so just double your antibody amount but then double as well your volume then you'll be almost in the same concentrations




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