Posted 16 October 2009 - 04:01 AM
Posted 16 October 2009 - 06:33 AM
You'll clearly need to do some sort of initial screening to select the most promising of the possible microRNA response elements (MRE) for more rigorous testing. I hope someone can suggest some in silico approaches for this (as I'd like to read those suggestions too!).
Once you have narrowed down the candidates, there are several ways to confirm the activity of a miRNA against a particular 3'-UTR. Some of these have been discussed in this thread:
I'm very curious to learn about approaches to this sort of problem -- you've many possible MRE to sort through.
Edited by Jon Moulton, 16 October 2009 - 06:37 AM.
Gene Tools, LLC
Posted 16 October 2009 - 10:17 AM