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Re: How to extract RNA fromFormalin fixed tissue


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7 replies to this topic

#1 anonymous

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Posted 08 March 2001 - 10:00 PM

I need a good protocol for nonradioactive in situ hybridizations. I will be using liver tissue sections.

#2 anonymous

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Posted 10 March 2001 - 10:00 PM

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#3 anonymous

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Posted 19 March 2001 - 10:00 PM

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#4 anonymous

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Posted 19 March 2001 - 10:00 PM

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#5 anonymous

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Posted 21 March 2001 - 10:00 PM

If you can specify what probe are you going to label and what nucleotide sequence are you going to detect I may provide you with some information

Longcheng LI


#6 anonymous

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Posted 21 March 2001 - 10:00 PM

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#7 anonymous

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Posted 22 March 2001 - 10:00 PM

Hi,

There is a great in depth article in the Boehringer (now Roche) newsletter, Biochemica. You can find it at:[url="http://biochem.boehringer-mannheim.com/Prod_Inf/Biochemi/no1_98/p10.pdf"][url]http://biochem.boehringer-mannheim.com/Pro.../no1_98/p10.pdf[/url][/url]

Another key reference is the one by Schaeren-Wiemers & Gerfin-Moser (1993) Histochemistry 100:431.

Good luck!

-Jonathan Lytton


#8 anonymous

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Posted 04 May 2001 - 09:00 PM

Check Clontech's SMART system. It is really reliable. Good luck




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