I need a good protocol for nonradioactive in situ hybridizations. I will be using liver tissue sections.
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#5
anonymous
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Posted 21 March 2001 - 10:00 PM
If you can specify what probe are you going to label and what nucleotide sequence are you going to detect I may provide you with some information
Longcheng LI
#7
anonymous
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Posted 22 March 2001 - 10:00 PM
Hi,
There is a great in depth article in the Boehringer (now Roche) newsletter, Biochemica. You can find it at:[url="http://biochem.boehringer-mannheim.com/Prod_Inf/Biochemi/no1_98/p10.pdf"][url]http://biochem.boehringer-mannheim.com/Pro.../no1_98/p10.pdf[/url][/url]
Another key reference is the one by Schaeren-Wiemers & Gerfin-Moser (1993) Histochemistry 100:431.
Good luck!
-Jonathan Lytton
#8
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Posted 04 May 2001 - 09:00 PM
Check Clontech's SMART system. It is really reliable. Good luck
